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Peptide Institute rat ghrelin
Rat Ghrelin, supplied by Peptide Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+ghrelin/ghrelin+rat/pmc12812557-68-0-2
Average 86 stars, based on 1 article reviews
rat ghrelin - by Bioz Stars, 2026-09
86/100 stars

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Article Title: Soy-fortelin: A ghrelin sensitivity-enhancing peptide that stimulates food intake in aged mice.
Article Snippet: Human ghrelin and rat ghrelin were obtained from Peptide Institute, Inc. (Osaka, Japan).

Article Title: Identification of pheasant ghrelin and motilin and their actions on contractility of the isolated gastrointestinal tract.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Dietary medium chain triglycerides impairs orexigenic action of ghrelin in mice
Article Snippet: Rat ghrelin (Peptide Institute, Osaka, Japan) solution of 0.18 μg/μl, 0.34 μg/μl, and 0.7 μg/μl were prepared for ghrelin infusion at rates of 0.9, 0.17, and 0.35 μg/hr, respectively and filled into a mini-osmotic pump (Alzet model 2001; Alza, Palo Alto, CA).

Article Title: Does ghrelin regulate intestinal motility in rabbits? An in vitro study using isolated duodenal strips.
Article Snippet: Rabbit duodenum has been used for examining the ability of motilin to cause muscle contraction in vitro.. A motilin-related peptide, ghrelin, is known to be involved in the regulation of gastrointestinal (GI) motility in various animals, but its ability to cause rabbit GI contraction have not been well examined.. The aim of this study is to clarify the action of rat ghrelin and its interaction with motilin in the rabbit duodenum.

Concentration Assay:

Article Title: Z-505 hydrochloride ameliorates chemotherapy-induced anorexia in rodents via activation of the ghrelin receptor, GHSR1a.
Article Snippet: Despite its therapeutic advantages, chemotherapy with anti-cancer drugs can cause adverse effects, including anorexia and weight loss.. Although most patients with cancer suffer from anorexia during chemotherapy, resulting in the need to suspend or cease treatment and thereby worsening prognosis, treatment options for anorexia remain limited.. Ghrelin is an orexigenic hormone that has been proposed to prevent anorexia.

Article Title: Preventives or remedies for hepatopathy
Article Snippet: A time flow of the present Example is shown in FIG. 1. (1) Preparation of DMN-Induced Hepatic Fibrosis/Liver Cirrhosis Model Upon use, a dilution in which DMN (manufactured by Wako Pure Chemical Industries, Ltd.) was diluted with a physiological saline solution (manufactured by Otsuka Pharmaceutical Co., Ltd., physiological saline solution described in the Japanese Pharmacopoeia) to 1% concentration (v/v) was prepared, and this was intraperitoneafly administered to male rats (4 weeks old) so that DMN became 1 mL/kg. .. Administration was performed once a day, for consecutive three days in one week, and administration was repeated for 6 weeks, and the first time administration date was regarded as day 1. (2) Preparation of Dosing Solution Rat ghrelin (purchased from Peptide Institute Inc., Japan) was added to 5 weight % mannitol as a solvent to a concentration of 1 mg/mL, this was dispensed every necessary amount per one time, and freezing-stored at about −80° C. until its use. ..

Solvent:

Article Title: Preventives or remedies for hepatopathy
Article Snippet: A time flow of the present Example is shown in FIG. 1. (1) Preparation of DMN-Induced Hepatic Fibrosis/Liver Cirrhosis Model Upon use, a dilution in which DMN (manufactured by Wako Pure Chemical Industries, Ltd.) was diluted with a physiological saline solution (manufactured by Otsuka Pharmaceutical Co., Ltd., physiological saline solution described in the Japanese Pharmacopoeia) to 1% concentration (v/v) was prepared, and this was intraperitoneafly administered to male rats (4 weeks old) so that DMN became 1 mL/kg. .. Administration was performed once a day, for consecutive three days in one week, and administration was repeated for 6 weeks, and the first time administration date was regarded as day 1. (2) Preparation of Dosing Solution Rat ghrelin (purchased from Peptide Institute Inc., Japan) was added to 5 weight % mannitol as a solvent to a concentration of 1 mg/mL, this was dispensed every necessary amount per one time, and freezing-stored at about −80° C. until its use. ..

Control:

Article Title: Ghrelin stimulates growth hormone release from the pituitary via hypothalamic growth hormone-releasing hormone neurons in the cichlid, Oreochromis niloticus.
Article Snippet: Ghrelin, a gut-brain peptide hormone, is implicated in a multiplicity of biological functions, including energy homeostasis and reproduction.. Neuronal systems that are involved in energy homeostasis as well as reproduction traverse the hypothalamus; however, the mechanism by which they control energy homeostasis is not fully understood.. The present study analyzes the anatomical relationship of neurons expressing gonadotropin-releasing hormone (GnRH), neuropeptide Y (NPY) and growth hormone-releasing hormone (GHRH) in a cichlid, tilapia (Oreochromis niloticus).

In Vitro:

Article Title: Structural determination, distribution, and physiological actions of ghrelin in the guinea pig.
Article Snippet: We identified guinea pig ghrelin (gp-ghrelin), and examined its distribution and physiological actions in the guinea-pig.. Gp-ghrelin is a 28-amino acid peptide (GASFR SPEHH SAQQR KESRK LPAKI QPR); seven amino acids are different from that of rat ghrelin at positions 2, 5, 10, 11, 19, 21, and 25, which include the conserved region known in mammals.. The third serine residue is mainly modified by n-decanoyl acid.



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Schematic illustration representing <t>ghrelin‐PEG‐AuNR</t> conjugation. The carboxyl groups in the C‐terminus or glutamic acid residues at positions 8 and 17 in acyl‐ghrelin are the potential targeted sites for NH 2 ‐PEG‐SH conjugation. Reaction conditions were optimized to favor the formation of mono‐PEG substituted ghrelin at its C‐terminus.
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Schematic illustration representing ghrelin‐PEG‐AuNR conjugation. The carboxyl groups in the C‐terminus or glutamic acid residues at positions 8 and 17 in acyl‐ghrelin are the potential targeted sites for NH 2 ‐PEG‐SH conjugation. Reaction conditions were optimized to favor the formation of mono‐PEG substituted ghrelin at its C‐terminus.

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Nose‐to‐Brain Delivery of Acyl‐Ghrelin Peptide Gold Nanoconjugates for Treatment of Neurodegenerative Diseases

doi: 10.1002/smll.202504517

Figure Lengend Snippet: Schematic illustration representing ghrelin‐PEG‐AuNR conjugation. The carboxyl groups in the C‐terminus or glutamic acid residues at positions 8 and 17 in acyl‐ghrelin are the potential targeted sites for NH 2 ‐PEG‐SH conjugation. Reaction conditions were optimized to favor the formation of mono‐PEG substituted ghrelin at its C‐terminus.

Article Snippet: Rat/Mouse Ghrelin (Total) ELISA kits and Rat/Mouse Ghrelin (Active) ELISA kits were purchased from Merck Life Science (UK).

Techniques: Conjugation Assay

Optimization of ghrelin‐PEG‐SH synthesis reaction conditions. A) SDS‐PAGE analysis of the reaction mixture containing 10 µg ghrelin by Western blotting analysis and B) iodine staining confirmed that a ghrelin‐to‐NH 2 ‐PEG‐SH molar ratio of 1:1 resulted in the highest yield of monofunctionalized ghrelin‐PEG, (ghrelin‐(PEG‐SH) 1 ).

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Nose‐to‐Brain Delivery of Acyl‐Ghrelin Peptide Gold Nanoconjugates for Treatment of Neurodegenerative Diseases

doi: 10.1002/smll.202504517

Figure Lengend Snippet: Optimization of ghrelin‐PEG‐SH synthesis reaction conditions. A) SDS‐PAGE analysis of the reaction mixture containing 10 µg ghrelin by Western blotting analysis and B) iodine staining confirmed that a ghrelin‐to‐NH 2 ‐PEG‐SH molar ratio of 1:1 resulted in the highest yield of monofunctionalized ghrelin‐PEG, (ghrelin‐(PEG‐SH) 1 ).

Article Snippet: Rat/Mouse Ghrelin (Total) ELISA kits and Rat/Mouse Ghrelin (Active) ELISA kits were purchased from Merck Life Science (UK).

Techniques: SDS Page, Western Blot, Staining

Physicochemical properties and biological evaluation of ghrelin‐PEG‐AuNRs and their precursors. A) Nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), dynamic light scattering. B) Dot blot immunostaining. C) 1 H NMR spectra analysis confirming the presence of PEG and ghrelin on AuNRs. D) Cytotoxicity of the AuNRs constructs in SN4741 cells at the 24 h timepoint using the modified LDH assay. Data is expressed as mean ± SD, n = 5. Altogether, ghrelin‐PEG‐AuNRs were successfully synthesized and demonstrated improved biocompatibility compared to as‐synthesized AuNRs.

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Nose‐to‐Brain Delivery of Acyl‐Ghrelin Peptide Gold Nanoconjugates for Treatment of Neurodegenerative Diseases

doi: 10.1002/smll.202504517

Figure Lengend Snippet: Physicochemical properties and biological evaluation of ghrelin‐PEG‐AuNRs and their precursors. A) Nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), dynamic light scattering. B) Dot blot immunostaining. C) 1 H NMR spectra analysis confirming the presence of PEG and ghrelin on AuNRs. D) Cytotoxicity of the AuNRs constructs in SN4741 cells at the 24 h timepoint using the modified LDH assay. Data is expressed as mean ± SD, n = 5. Altogether, ghrelin‐PEG‐AuNRs were successfully synthesized and demonstrated improved biocompatibility compared to as‐synthesized AuNRs.

Article Snippet: Rat/Mouse Ghrelin (Total) ELISA kits and Rat/Mouse Ghrelin (Active) ELISA kits were purchased from Merck Life Science (UK).

Techniques: Transmission Assay, Electron Microscopy, Dot Blot, Immunostaining, Construct, Modification, Lactate Dehydrogenase Assay, Synthesized

Brain uptake and bioactivity of ghrelin‐PEG‐AuNRs after intranasal administration. C57BL/6 mice were intranasally administered with ghrelin‐PEG‐AuNRs at A) 10 or 20 µg ghrelin equivalent and sacrificed at 10 min post‐administration or B) 10 µg ghrelin equivalent and sacrificed at 10, 30, or 60 min post‐administration. Tissue lysates were analyzed using the total or active ELISA kits for quantification of total and acyl‐ghrelin, respectively. C) Ghrelin‐PEG‐AuNRs induced AMPK phosphorylation at 30 and 60 min post‐administration as evidenced by the representative Western blot images of pAMPKα and AMPKα and quantification of pAMPKα/AMPKα levels in brain lysates. Values were expressed as mean ± SD, n = 3. * p < 0.05, ** p < 0.01.

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Nose‐to‐Brain Delivery of Acyl‐Ghrelin Peptide Gold Nanoconjugates for Treatment of Neurodegenerative Diseases

doi: 10.1002/smll.202504517

Figure Lengend Snippet: Brain uptake and bioactivity of ghrelin‐PEG‐AuNRs after intranasal administration. C57BL/6 mice were intranasally administered with ghrelin‐PEG‐AuNRs at A) 10 or 20 µg ghrelin equivalent and sacrificed at 10 min post‐administration or B) 10 µg ghrelin equivalent and sacrificed at 10, 30, or 60 min post‐administration. Tissue lysates were analyzed using the total or active ELISA kits for quantification of total and acyl‐ghrelin, respectively. C) Ghrelin‐PEG‐AuNRs induced AMPK phosphorylation at 30 and 60 min post‐administration as evidenced by the representative Western blot images of pAMPKα and AMPKα and quantification of pAMPKα/AMPKα levels in brain lysates. Values were expressed as mean ± SD, n = 3. * p < 0.05, ** p < 0.01.

Article Snippet: Rat/Mouse Ghrelin (Total) ELISA kits and Rat/Mouse Ghrelin (Active) ELISA kits were purchased from Merck Life Science (UK).

Techniques: Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Western Blot